@Rowan-Dempster , you should use a monochrome stream (_grey), since QVIO needs a RAW8 image.
If you are not using MISP on hires cameras, that is fine, you can start off using the output of the ISP.
You should calibrate the camera using whatever resolution you decide to try. This is to avoid any confusion, since if you using ISP pipeline, the camera pipeline may select a higher resolution and downscale + crop. So whenever you are changing resolutions, it is always good to do a quick camera calibration to confirm the camera parameters.
When using MISP, we have more control over which camera mode is selected, because MISP gets the RAW data, not processed by the ISP, so we know the exact dimensions of the image sent from camera.
Alex